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Injuring DLMs by a single pinprick at PS (presutural) region
DOI: 10.21769/BioProtoc.v317 Published: May 20, 2018 Views: 4552
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Injuring DLMs by a single pinprick at PS (presutural) region. For demonstration purpose, the fly was immobilized using a little drop of nail polish on a glass slide. During actual experiments use of any adhesive is unnecessary. As shown in the video, we make a single prick at PS (presutural) location highlighted with a red arrow (Also shown in Figure 1A). To minimize damage to surrounding tissue, it is crucial to be quick and strictly to avoid moving the pin once it pierces fly thorax. This video was acquired using Stereo microscope coupled with a camera (see Equipment section for technical details). For image acquisition, an open source software micromanager (μManager) has been used. For further reference see Edelstein et al., 2014. Images and videos were processed using Fiji.
Extracted from protocol
A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila
Chakraborty, K., VijayRaghavan, K. and Gunage, R. (2018). Bio-protocol 8(10): e2860.
DOI: 10.21769/BioProtoc.2860.
Injuring DLMs by a single pinprick at PS (presutural) region. For demonstration purpose, the fly was immobilized using a little drop of nail polish on a glass slide. During actual experiments use of any adhesive is unnecessary. As shown in the video, we make a single prick at PS (presutural) location highlighted with a red arrow (Also shown in Figure 1A). To minimize damage to surrounding tissue, it is crucial to be quick and strictly to avoid moving the pin once it pierces fly thorax. This video was acquired using Stereo microscope coupled with a camera (see Equipment section for technical details). For image acquisition, an open source software micromanager (μManager) has been used. For further reference see Edelstein et al., 2014. Images and videos were processed using Fiji.
Extracted from protocol
A Method to Injure, Dissect and Image Indirect Flight Muscle of Drosophila
Chakraborty, K., VijayRaghavan, K. and Gunage, R. (2018). Bio-protocol 8(10): e2860.
DOI: 10.21769/BioProtoc.2860.
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Keywords
Drosophila flight muscles, Injury, Regeneration, Insect satellite cells
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