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Fly head dissection.
DOI: 10.21769/BioProtoc.v211 Published: Jan 20, 2019 Views: 3177
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First, a glass homogenizer containing 50 µl homogenization buffer is put on ice. Flies are anesthetized with CO2 in the vial for a few seconds. Once the flies are immobile, they are transferred to a PDMS dissection plate, which is placed on top of a cold plate. Using a pair of fine forceps the abdomen is hold, and the head is gently pinched off with a second pair of forceps or fine scissors. Finally, the head is directly transferred into the glass homogenizer containing homogenization buffer. Continue until a total of 20 dissected heads are collected in the homogenizer.
Extracted from protocol
Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila
Marter, K., Kobler, O., Erdmann, I., Soleimanpour, E., Landgraf, P., Müller, A., Abele, J., Thomas, U. and Dieterich, D. C. (2019). Bio-protocol 9(2): e3142.
DOI: 10.21769/BioProtoc.3142.
First, a glass homogenizer containing 50 µl homogenization buffer is put on ice. Flies are anesthetized with CO2 in the vial for a few seconds. Once the flies are immobile, they are transferred to a PDMS dissection plate, which is placed on top of a cold plate. Using a pair of fine forceps the abdomen is hold, and the head is gently pinched off with a second pair of forceps or fine scissors. Finally, the head is directly transferred into the glass homogenizer containing homogenization buffer. Continue until a total of 20 dissected heads are collected in the homogenizer.
Extracted from protocol
Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila
Marter, K., Kobler, O., Erdmann, I., Soleimanpour, E., Landgraf, P., Müller, A., Abele, J., Thomas, U. and Dieterich, D. C. (2019). Bio-protocol 9(2): e3142.
DOI: 10.21769/BioProtoc.3142.
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Keywords
Bio-orthogonal chemical reporters, Click chemistry, CuAAC, Protein tagging, Drosophila melanogaster, Proteomic profiling, Protein synthesis
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