Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Larval body wall dissection at 3-fold time-lapse.
DOI: 10.21769/BioProtoc.v210 Published: Jan 20, 2019 Views: 3196
Log in to your account to watch the full video
Video legend
A wet larva is placed on the dissection chamber shown in the supplementary figure. Using the central, straight clips the animal is immobilized and moderately stretched, dorsal side up. The animal is then covered with Ca2+-free saline. Using the spring scissors a hole is squeezed in at about 75% length, serving as a starting point for cutting along the dorsal midline, first direction mouth hook and then towards the posterior end. Short incisions are made to the right and the left at either end. To avoid scratching the body wall muscles and harming the larval brain with the blades, the scissors are slightly lifted during the cutting. Once cut, the larva is pushed a bit to squeeze out inner organs such as the gut and fat body and thus to ease their removal. The lateral clips are then used to carefully span the body wall.
Extracted from protocol
Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila
Marter, K., Kobler, O., Erdmann, I., Soleimanpour, E., Landgraf, P., Müller, A., Abele, J., Thomas, U. and Dieterich, D. C. (2019). Bio-protocol 9(2): e3142.
DOI: 10.21769/BioProtoc.3142.
A wet larva is placed on the dissection chamber shown in the supplementary figure. Using the central, straight clips the animal is immobilized and moderately stretched, dorsal side up. The animal is then covered with Ca2+-free saline. Using the spring scissors a hole is squeezed in at about 75% length, serving as a starting point for cutting along the dorsal midline, first direction mouth hook and then towards the posterior end. Short incisions are made to the right and the left at either end. To avoid scratching the body wall muscles and harming the larval brain with the blades, the scissors are slightly lifted during the cutting. Once cut, the larva is pushed a bit to squeeze out inner organs such as the gut and fat body and thus to ease their removal. The lateral clips are then used to carefully span the body wall.
Extracted from protocol
Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila
Marter, K., Kobler, O., Erdmann, I., Soleimanpour, E., Landgraf, P., Müller, A., Abele, J., Thomas, U. and Dieterich, D. C. (2019). Bio-protocol 9(2): e3142.
DOI: 10.21769/BioProtoc.3142.
Q&A
Favorite
Keywords
Bio-orthogonal chemical reporters, Click chemistry, CuAAC, Protein tagging, Drosophila melanogaster, Proteomic profiling, Protein synthesis
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
0/150
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question0 Q&A
