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Preparing imaging chamber for microscopy.
DOI: 10.21769/BioProtoc.v205 Published: Dec 5, 2018 Views: 2360
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Video legend
Part 1, a video clip showing Steps 1-3 of Procedure B. Part 2, a video clip showing how to pipet solution into the imaging chamber. Part 3, a video clip demonstrating how to replace the solution in the imaging chamber. For visualization purpose, PEM buffer spiked with bromophenol blue was used in the demonstration shown in the clips for Parts 2 and 3.
Extracted from protocol
An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Zhu, Y., Tan, W. and Lee, W. (2018). Bio-protocol 8(23): e3110.
DOI: 10.21769/BioProtoc.3110.
Part 1, a video clip showing Steps 1-3 of Procedure B. Part 2, a video clip showing how to pipet solution into the imaging chamber. Part 3, a video clip demonstrating how to replace the solution in the imaging chamber. For visualization purpose, PEM buffer spiked with bromophenol blue was used in the demonstration shown in the clips for Parts 2 and 3.
Extracted from protocol
An in vitro Microscopy-based Assay for Microtubule-binding and Microtubule-crosslinking by Budding Yeast Microtubule-associated Protein
Zhu, Y., Tan, W. and Lee, W. (2018). Bio-protocol 8(23): e3110.
DOI: 10.21769/BioProtoc.3110.
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Keywords
Microtubule, Yeast, Microtubule-associated protein, Microtubule binding, Microtubule crosslinking, Microscopy-based assay, Imaging chamber
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