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Imaging chamber preparation and sample mounting.
DOI: 10.21769/BioProtoc.v190 Published: Oct 20, 2018 Views: 4401
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Cut a silicone rubber sheet (1 mm-thick and 0.2 mm-thick are shown in this video) into an appropriate size with scissors or a utility knife. A silicone rubber sheet will adhere to a glass slide, by removing air between the rubber sheet and the glass slide. Mount cleared samples (cleared samples are often difficult to find) using a perforated spoon and a paint brush. Use No. 1.5H coverslips to seal the samples.
Extracted from protocol
Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2
Ke, M. and Imai, T. (2018). Bio-protocol 8(20): e3046.
DOI: 10.21769/BioProtoc.3046.
Cut a silicone rubber sheet (1 mm-thick and 0.2 mm-thick are shown in this video) into an appropriate size with scissors or a utility knife. A silicone rubber sheet will adhere to a glass slide, by removing air between the rubber sheet and the glass slide. Mount cleared samples (cleared samples are often difficult to find) using a perforated spoon and a paint brush. Use No. 1.5H coverslips to seal the samples.
Extracted from protocol
Optical Clearing and Index Matching of Tissue Samples for High-resolution Fluorescence Imaging Using SeeDB2
Ke, M. and Imai, T. (2018). Bio-protocol 8(20): e3046.
DOI: 10.21769/BioProtoc.3046.
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Keywords
Tissue clearing, Fluorescence imaging, Confocal imaging, Super-resolution imaging, Connectome, SeeDB2
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