Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
FACS Diva acquisition Non-Transfected negative control.
DOI: 10.21769/BioProtoc.v181 Published: Sep 5, 2018 Views: 4893
Log in to your account to watch the full video
Video legend
Panels show the gates used for HEK293 cells: scatter plots for singlets (FSC.A vs. FSC.H), cell population (FSC.A vs SSC.A), histograms and scatter plot for PE.A (550LP-BP575/26), and the analytical output for CFP and YFP (Scatter plot PE.A vs. Pacific blue).
Extracted from protocol
Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells
Lemus-Diaz, N., Tamon, L. and Gruber, J. (2018). Bio-protocol 8(17): e3000.
DOI: 10.21769/BioProtoc.3000.
Panels show the gates used for HEK293 cells: scatter plots for singlets (FSC.A vs. FSC.H), cell population (FSC.A vs SSC.A), histograms and scatter plot for PE.A (550LP-BP575/26), and the analytical output for CFP and YFP (Scatter plot PE.A vs. Pacific blue).
Extracted from protocol
Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells
Lemus-Diaz, N., Tamon, L. and Gruber, J. (2018). Bio-protocol 8(17): e3000.
DOI: 10.21769/BioProtoc.3000.
Q&A
Favorite
Keywords
miRNA, Flow-Cytometry, Dual fluorescence reporter, Functional assay, ncRNA, Small RNA, non-protein-coding RNA, Gene regulation, Reporter gene system, Single cell analysis, Flow cytometry
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
0/150
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question0 Q&A
