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FACS Diva acquisition Non-Transfected negative control.
DOI: 10.21769/BioProtoc.v181 Published: Sep 5, 2018 Views: 4929
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Panels show the gates used for HEK293 cells: scatter plots for singlets (FSC.A vs. FSC.H), cell population (FSC.A vs SSC.A), histograms and scatter plot for PE.A (550LP-BP575/26), and the analytical output for CFP and YFP (Scatter plot PE.A vs. Pacific blue).
Extracted from protocol
Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells
Lemus-Diaz, N., Tamon, L. and Gruber, J. (2018). Bio-protocol 8(17): e3000.
DOI: 10.21769/BioProtoc.3000.
Panels show the gates used for HEK293 cells: scatter plots for singlets (FSC.A vs. FSC.H), cell population (FSC.A vs SSC.A), histograms and scatter plot for PE.A (550LP-BP575/26), and the analytical output for CFP and YFP (Scatter plot PE.A vs. Pacific blue).
Extracted from protocol
Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells
Lemus-Diaz, N., Tamon, L. and Gruber, J. (2018). Bio-protocol 8(17): e3000.
DOI: 10.21769/BioProtoc.3000.
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Keywords
miRNA, Flow-Cytometry, Dual fluorescence reporter, Functional assay, ncRNA, Small RNA, non-protein-coding RNA, Gene regulation, Reporter gene system, Single cell analysis, Flow cytometry
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