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Mixing of spheroids and collagen.
DOI: 10.21769/BioProtoc.v180 Published: Sep 5, 2018 Views: 6238
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Spheroids are suspended in methocel containing 20 % FBS and mixed with collagen. Solution is added to wells of a 24-well plate.
Extracted from protocol
Human Endothelial Cell Spheroid-based Sprouting Angiogenesis Assay in Collagen
Tetzlaff, F. and Fischer, A. (2018). Bio-protocol 8(17): e2995.
DOI: 10.21769/BioProtoc.2995.
Spheroids are suspended in methocel containing 20 % FBS and mixed with collagen. Solution is added to wells of a 24-well plate.
Extracted from protocol
Human Endothelial Cell Spheroid-based Sprouting Angiogenesis Assay in Collagen
Tetzlaff, F. and Fischer, A. (2018). Bio-protocol 8(17): e2995.
DOI: 10.21769/BioProtoc.2995.
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Keywords
Endothelial cells, Angiogenesis, 3D cell culture, Capillary sprout formation, Spheroids, Vascular
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