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Gel electroelution protocol.
DOI: 10.21769/BioProtoc.v173 Published: Sep 5, 2018 Views: 1839
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This video illustrates the procedure of gel electroelution described above in Procedure C (PolyU Purification). The video illustrates Step C4, wherein the portion of the gel containing the desired RNA is excised and the RNA is then electroeluted out of this band into TAE buffer in dialysis tubing.
Extracted from protocol
Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs
Beren, C., Liu, K. N., Dreesens, L. L., Knobler, C. M. and Gelbart, W. M. (2018). Bio-protocol 8(17): e2988.
DOI: 10.21769/BioProtoc.2988.
This video illustrates the procedure of gel electroelution described above in Procedure C (PolyU Purification). The video illustrates Step C4, wherein the portion of the gel containing the desired RNA is excised and the RNA is then electroeluted out of this band into TAE buffer in dialysis tubing.
Extracted from protocol
Enzymatic Synthesis and Fractionation of Fluorescent PolyU RNAs
Beren, C., Liu, K. N., Dreesens, L. L., Knobler, C. M. and Gelbart, W. M. (2018). Bio-protocol 8(17): e2988.
DOI: 10.21769/BioProtoc.2988.
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Keywords
Homopolymer polyU RNA, Gel electrophoresis electroelution, Dynamic light scattering, RNA secondary structure, Viral RNA
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