Setting up the plate reader

K Kanamori et al.

DOI: 10.21769/BioProtoc.v165 Published: Jul 20, 2018 Views: 2807

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Setting up the plate reader

Extracted from protocol
Two Different Methods of Quantification of Oxidized Nicotinamide Adenine Dinucleotide (NAD+) and Reduced Nicotinamide Adenine Dinucleotide (NADH) Intracellular Levels: Enzymatic Coupled Cycling Assay and Ultra-performance Liquid Chromatography (UPLC)-Mass Spectrometry
Kanamori, K. S., de Oliveira, G. C., Auxiliadora-Martins, M., Schoon, R. A., Reid, J. M. and Chini, E. N. (2018). Bio-protocol 8(14): e2937.
DOI: 10.21769/BioProtoc.2937.

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Keywords

NAD+ levels, NADH, Cycling assay, NAD+ degradation, Fluorescence, Mass spectroscopy, Aging, Metabolism, UPLC

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