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Analysis of in situ zymography assay
DOI: 10.21769/BioProtoc.v158 Published: May 20, 2018 Views: 3348
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Analysis of in situ zymography assay. Open images acquired at the end of each experiment (both bright-field and fluorescent images) in ImageJ. Count the total number of follicles using the multi-point tool in the bright-field image. In this example, there are a total of 29 follicles. Then, count the total number of follicles with posterior green fluorescence in the green-fluorescent image. Before taking the image, most of the follicles identified with enzymatic activity were segregated toward the top of the image. In this example, there are a total of 12 follicles with posterior green fluorescence; therefore, 41.4% of follicles had enzymatic activity.
Extracted from protocol
Ex vivo Follicle Rupture and in situ Zymography in Drosophila
Knapp, E. M., Deady, L. D. and Sun, J. (2018). Bio-protocol 8(10): e2846.
DOI: 10.21769/BioProtoc.2846.
Analysis of in situ zymography assay. Open images acquired at the end of each experiment (both bright-field and fluorescent images) in ImageJ. Count the total number of follicles using the multi-point tool in the bright-field image. In this example, there are a total of 29 follicles. Then, count the total number of follicles with posterior green fluorescence in the green-fluorescent image. Before taking the image, most of the follicles identified with enzymatic activity were segregated toward the top of the image. In this example, there are a total of 12 follicles with posterior green fluorescence; therefore, 41.4% of follicles had enzymatic activity.
Extracted from protocol
Ex vivo Follicle Rupture and in situ Zymography in Drosophila
Knapp, E. M., Deady, L. D. and Sun, J. (2018). Bio-protocol 8(10): e2846.
DOI: 10.21769/BioProtoc.2846.
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Keywords
Drosophila, Ovulation, Follicle rupture, Octopamine, in situ zymography, Follicle cells
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