Improve Research Reproducibility A Bio-protocol resource
- Protocols
- About
-
Analysis of in situ zymography assay
DOI: 10.21769/BioProtoc.v158 Published: May 20, 2018 Views: 3403
Log in to your account to watch the full video
Video legend
Analysis of in situ zymography assay. Open images acquired at the end of each experiment (both bright-field and fluorescent images) in ImageJ. Count the total number of follicles using the multi-point tool in the bright-field image. In this example, there are a total of 29 follicles. Then, count the total number of follicles with posterior green fluorescence in the green-fluorescent image. Before taking the image, most of the follicles identified with enzymatic activity were segregated toward the top of the image. In this example, there are a total of 12 follicles with posterior green fluorescence; therefore, 41.4% of follicles had enzymatic activity.
Extracted from protocol
Ex vivo Follicle Rupture and in situ Zymography in Drosophila
Knapp, E. M., Deady, L. D. and Sun, J. (2018). Bio-protocol 8(10): e2846.
DOI: 10.21769/BioProtoc.2846.
Analysis of in situ zymography assay. Open images acquired at the end of each experiment (both bright-field and fluorescent images) in ImageJ. Count the total number of follicles using the multi-point tool in the bright-field image. In this example, there are a total of 29 follicles. Then, count the total number of follicles with posterior green fluorescence in the green-fluorescent image. Before taking the image, most of the follicles identified with enzymatic activity were segregated toward the top of the image. In this example, there are a total of 12 follicles with posterior green fluorescence; therefore, 41.4% of follicles had enzymatic activity.
Extracted from protocol
Ex vivo Follicle Rupture and in situ Zymography in Drosophila
Knapp, E. M., Deady, L. D. and Sun, J. (2018). Bio-protocol 8(10): e2846.
DOI: 10.21769/BioProtoc.2846.
Q&A
Favorite
Keywords
Drosophila, Ovulation, Follicle rupture, Octopamine, in situ zymography, Follicle cells
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
0/150
+
Post a Question0 Q&A
