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Isolation of the leech nervous system (a short chain of ganglia).
DOI: 10.21769/BioProtoc.v150 Published: Mar 5, 2018 Views: 3604
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1. Anesthetize a leech in ice-cold saline. 2. Pin down the leech’s head and tail, using insect pins on a coarse dissection container. 3. Cut the dorsal skin along with the midline with a pair of dissection scissors. 4. Cut open the skin and pin it down. 5. Find a target ganglion for imaging. 6. Use a microsurgical knife to expose a lateral nerve root to be extracellularly recorded, if needed. 7. Dissect away the blood sinus surrounding the target ganglion. 8. Dissect away the blood sinus with the lateral nerves of the ganglion. 9. Isolate a short chain of ganglia. 10. Aspirate the isolated preparation into a pipette.
Extracted from protocol
Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia
Tomina, Y. and Wagenaar, D. A. (2018). Bio-protocol 8(5): e2751.
DOI: 10.21769/BioProtoc.2751.
1. Anesthetize a leech in ice-cold saline. 2. Pin down the leech’s head and tail, using insect pins on a coarse dissection container. 3. Cut the dorsal skin along with the midline with a pair of dissection scissors. 4. Cut open the skin and pin it down. 5. Find a target ganglion for imaging. 6. Use a microsurgical knife to expose a lateral nerve root to be extracellularly recorded, if needed. 7. Dissect away the blood sinus surrounding the target ganglion. 8. Dissect away the blood sinus with the lateral nerves of the ganglion. 9. Isolate a short chain of ganglia. 10. Aspirate the isolated preparation into a pipette.
Extracted from protocol
Dual-sided Voltage-sensitive Dye Imaging of Leech Ganglia
Tomina, Y. and Wagenaar, D. A. (2018). Bio-protocol 8(5): e2751.
DOI: 10.21769/BioProtoc.2751.
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Keywords
Voltage-sensitive dye imaging, Whole-brain recording, VoltageFluor, Microdissection, Dye-loading process
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