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A short tutorial (~20 min) on how to use ImageJ and R to establish single molecule sensitivity of an smFISH experiment.
DOI: 10.21769/BioProtoc.v149 Published: Mar 5, 2018 Views: 5494
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A further tutorial on using the xsPT plugi-in is available in Gaspar et al., 2017.
Extracted from protocol
Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture
Gáspár, I., Wippich, F. and Ephrussi, A. (2018). Bio-protocol 8(5): e2750.
DOI: 10.21769/BioProtoc.2750.
A further tutorial on using the xsPT plugi-in is available in Gaspar et al., 2017.
Extracted from protocol
Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture
Gáspár, I., Wippich, F. and Ephrussi, A. (2018). Bio-protocol 8(5): e2750.
DOI: 10.21769/BioProtoc.2750.
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Keywords
Terminal deoxynucleotidyl transferase, Labelled terminator nucleotide, Probe production, Single molecule FISH (smFISH), RNA capture, RNA affinity purification (RAP)
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