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Collection of muscles for SC isolation.
DOI: 10.21769/BioProtoc.v141 Published: Jan 20, 2018 Views: 4938
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After skin removal, hindlimb muscles and diaphragm are harvested from the GFP donor mouse and collected in pre-warmed serum-free DMEM.
Extracted from protocol
Mouse Satellite Cell Isolation and Transplantation
Angelino, E., Reano, S., Ferrara, M., Agosti, E., Sustova, H., Malacarne, V., Clerici, S., Graziani, A. and Filigheddu, N. (2018). Bio-protocol 8(2): e2696.
DOI: 10.21769/BioProtoc.2696.
After skin removal, hindlimb muscles and diaphragm are harvested from the GFP donor mouse and collected in pre-warmed serum-free DMEM.
Extracted from protocol
Mouse Satellite Cell Isolation and Transplantation
Angelino, E., Reano, S., Ferrara, M., Agosti, E., Sustova, H., Malacarne, V., Clerici, S., Graziani, A. and Filigheddu, N. (2018). Bio-protocol 8(2): e2696.
DOI: 10.21769/BioProtoc.2696.
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Keywords
Satellite cells, Muscle stem cells, Transplant, Engraftment, Skeletal muscle, Regeneration, Muscle excision, Cardiotoxin
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