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Preparation of the recipient muscle.
DOI: 10.21769/BioProtoc.v140 Published: Jan 20, 2018 Views: 4938
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After removing the hairs from the front of the limb, cardiotoxin (CTX) is injected in the mid-belly region of the tibialis anterior (TA) muscle of the anesthetized mouse using a Hamilton repeating dispenser.
Extracted from protocol
Mouse Satellite Cell Isolation and Transplantation
Angelino, E., Reano, S., Ferrara, M., Agosti, E., Sustova, H., Malacarne, V., Clerici, S., Graziani, A. and Filigheddu, N. (2018). Bio-protocol 8(2): e2696.
DOI: 10.21769/BioProtoc.2696.
After removing the hairs from the front of the limb, cardiotoxin (CTX) is injected in the mid-belly region of the tibialis anterior (TA) muscle of the anesthetized mouse using a Hamilton repeating dispenser.
Extracted from protocol
Mouse Satellite Cell Isolation and Transplantation
Angelino, E., Reano, S., Ferrara, M., Agosti, E., Sustova, H., Malacarne, V., Clerici, S., Graziani, A. and Filigheddu, N. (2018). Bio-protocol 8(2): e2696.
DOI: 10.21769/BioProtoc.2696.
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Keywords
Satellite cells, Muscle stem cells, Transplant, Engraftment, Skeletal muscle, Regeneration, Muscle excision, Cardiotoxin
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