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Grid filming procedure.
DOI: 10.21769/BioProtoc.v135 Published: Jan 5, 2018 Views: 4503
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1- Glass Pasteur pipet and its pump; 2- Petri dish; 3- Glass wand; 4- Large Petri dish lid; 5- Whatman filter paper type 41; 6- Crystallizer; 7- EM grids. 8- 2% parlodion solution; 9- Type 5X tweezers.
Extracted from protocol
Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
Nicolas, W. J., Bayer, E. and Brocard, L. (2018). Bio-protocol 8(1): e2681.
DOI: 10.21769/BioProtoc.2681.
1- Glass Pasteur pipet and its pump; 2- Petri dish; 3- Glass wand; 4- Large Petri dish lid; 5- Whatman filter paper type 41; 6- Crystallizer; 7- EM grids. 8- 2% parlodion solution; 9- Type 5X tweezers.
Extracted from protocol
Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
Nicolas, W. J., Bayer, E. and Brocard, L. (2018). Bio-protocol 8(1): e2681.
DOI: 10.21769/BioProtoc.2681.
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Keywords
Plasmodesmata, Plant, Cell wall, Electron tomography, Electron microscopy, Cryofixation
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