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Loading the biological sample in the HPF pods.
DOI: 10.21769/BioProtoc.v132 Published: Jan 5, 2018 Views: 4516
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(1) 200 µm deep membrane carriers (Leica Microsystems); (2) Vertically grown Arabidopsis seedlings; (3) Becker filled liquid MS medium; (4) 20% BSA diluted in liquid MS medium; (5) 0.5-2 µl and 20-200 µl range pipets (Sartorius); (6) Torque screwdriver set to 30Ncm (TOHNICHI, Torque driver RTD60CN); (7) Disposable razor blades; (8) Style 7 EMS tweezers; (9) Disposable tooth picks; (10) Leica loading system (with rod), pods, pod holder.
Extracted from protocol
Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
Nicolas, W. J., Bayer, E. and Brocard, L. (2018). Bio-protocol 8(1): e2681.
DOI: 10.21769/BioProtoc.2681.
(1) 200 µm deep membrane carriers (Leica Microsystems); (2) Vertically grown Arabidopsis seedlings; (3) Becker filled liquid MS medium; (4) 20% BSA diluted in liquid MS medium; (5) 0.5-2 µl and 20-200 µl range pipets (Sartorius); (6) Torque screwdriver set to 30Ncm (TOHNICHI, Torque driver RTD60CN); (7) Disposable razor blades; (8) Style 7 EMS tweezers; (9) Disposable tooth picks; (10) Leica loading system (with rod), pods, pod holder.
Extracted from protocol
Electron Tomography to Study the Three-dimensional Structure of Plasmodesmata in Plant Tissues–from High Pressure Freezing Preparation to Ultrathin Section Collection
Nicolas, W. J., Bayer, E. and Brocard, L. (2018). Bio-protocol 8(1): e2681.
DOI: 10.21769/BioProtoc.2681.
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Keywords
Plasmodesmata, Plant, Cell wall, Electron tomography, Electron microscopy, Cryofixation
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