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Dissection of mouse skin after Arthus reaction.
DOI: 10.21769/BioProtoc.v123 Published: Dec 20, 2017 Views: 3694
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The Arthus reaction was elicited by i.p. injection of 100 µl PBS solution containing 1% Evans blue and 2% BSA. Afterward, 40 µl of anti-BSA antibody (left-side of back-skin) or 40 µl rabbit serum (right-side of back-skin) was intradermally injected. 4 h later, mice were sacrificed and the skin specimen was harvested and digitally photographed.
Extracted from protocol
Mouse Model of Immune Complex-mediated Vasculitis in Dorsal Skin and Assessment of the Neutrophil-mediated Tissue Damage
Jiang, D., de Vries, J. C., Muschhammer, J., Sindrilaru, A. and Scharffetter-Kochanek, K. (2017). Bio-protocol 7(24): e2660.
DOI: 10.21769/BioProtoc.2660.
The Arthus reaction was elicited by i.p. injection of 100 µl PBS solution containing 1% Evans blue and 2% BSA. Afterward, 40 µl of anti-BSA antibody (left-side of back-skin) or 40 µl rabbit serum (right-side of back-skin) was intradermally injected. 4 h later, mice were sacrificed and the skin specimen was harvested and digitally photographed.
Extracted from protocol
Mouse Model of Immune Complex-mediated Vasculitis in Dorsal Skin and Assessment of the Neutrophil-mediated Tissue Damage
Jiang, D., de Vries, J. C., Muschhammer, J., Sindrilaru, A. and Scharffetter-Kochanek, K. (2017). Bio-protocol 7(24): e2660.
DOI: 10.21769/BioProtoc.2660.
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Keywords
Vasculitis, Neutrophil, Evans blue, Tissue damage
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