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Elimination of extracellular debris using Percoll solution.
DOI: 10.21769/BioProtoc.v122 Published: Dec 20, 2017 Views: 6360
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The cell suspension is carefully layered onto a 22% cold Percoll solution in a continuous drop-by-drop manner, with the tube being tilted at 45°, minimizing contact between the Percoll solution and the cell suspension. This step allows elimination of myelin and other extracellular debris.
Extracted from protocol
FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
Tome-Garcia, J., Doetsch, F. and Tsankova, N. M. (2017). Bio-protocol 7(24): e2659.
DOI: 10.21769/BioProtoc.2659.
The cell suspension is carefully layered onto a 22% cold Percoll solution in a continuous drop-by-drop manner, with the tube being tilted at 45°, minimizing contact between the Percoll solution and the cell suspension. This step allows elimination of myelin and other extracellular debris.
Extracted from protocol
FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
Tome-Garcia, J., Doetsch, F. and Tsankova, N. M. (2017). Bio-protocol 7(24): e2659.
DOI: 10.21769/BioProtoc.2659.
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Keywords
EGF, EGFR, FACS, Stem cells, Neural, Glioblastoma, Germinal matrix, Human
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