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Primary trituration of dissociated tissue suspension using P1000.
DOI: 10.21769/BioProtoc.v120 Published: Dec 20, 2017 Views: 6634
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After enzymatic digestion, the dissociated cell pellet is triturated continuously first with a P1000 pipette until no large tissue chunks are visible.
Extracted from protocol
FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
Tome-Garcia, J., Doetsch, F. and Tsankova, N. M. (2017). Bio-protocol 7(24): e2659.
DOI: 10.21769/BioProtoc.2659.
After enzymatic digestion, the dissociated cell pellet is triturated continuously first with a P1000 pipette until no large tissue chunks are visible.
Extracted from protocol
FACS-based Isolation of Neural and Glioma Stem Cell Populations from Fresh Human Tissues Utilizing EGF Ligand
Tome-Garcia, J., Doetsch, F. and Tsankova, N. M. (2017). Bio-protocol 7(24): e2659.
DOI: 10.21769/BioProtoc.2659.
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Keywords
EGF, EGFR, FACS, Stem cells, Neural, Glioblastoma, Germinal matrix, Human
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