Transfer the epidermal fragments by pouring the solution onto the fragments on the mesh using a wash bottle (or 10 ml Komagome pipette, not shown here)

Yamauchi et al.

doi:10.21769/BioProtoc.v117

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Transfer the epidermal fragments by pouring the solution onto the fragments on the mesh using a wash bottle (or 10 ml Komagome pipette, not shown here)

Y Yamauchi et al.

DOI: 10.21769/BioProtoc.v117 Published: Dec 20, 2017 Views: 4338

Extracted from protocol
Determination of H⁺-ATPase Activity in Arabidopsis Guard Cell Protoplasts through H⁺-pumping Measurement and H⁺-ATPase Quantification
Yamauchi, S. and Shimazaki, K. (2017). Bio-protocol 7(24): e2653.
DOI: 10.21769/BioProtoc.2653.

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Keywords

Arabidopsis thaliana, Blue light, Fusicoccin, H⁺-pumping, Plasma membrane H⁺-ATPase

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