Injection of flies.

O Okuda et al.

DOI: 10.21769/BioProtoc.v116 Published: Dec 5, 2017 Views: 3719

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This video demonstrates the injection of 32 nl of a 1% bromophenol blue solution (for better visualization) in adult flies using a microinjector. The dye solution enters the hemocoel and expands the abdomen.

Extracted from protocol
Drosophila Model of Leishmania amazonensis Infection
Okuda, K. and Silverman, N. (2017). Bio-protocol 7(23): e2640.
DOI: 10.21769/BioProtoc.2640.

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Keywords

Drosophila melanogaster, Leishmania, Phagocytosis, Plasmatocytes, Hemocytes, Axenic amastigote, Amastigote, RNAi screen

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