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Mounting of sections on a slide.
DOI: 10.21769/BioProtoc.v114 Published: Nov 20, 2017 Views: 7074
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Visual demonstration of mounting gelatin embedded brain slices in a weigh boat filled with 0.12% w/v gelatin. In this example, only one of four vials was used for mapping antibody-mediated toxin deletions (each slice is 240 μm apart) and sequentially arranged from top left, moving along top to bottom, left to right.
Extracted from protocol
A Streamlined Method for the Preparation of Gelatin Embedded Brains and Simplified Organization of Sections for Serial Reconstructions
Liu, A. W., Aoki, S. and Wickens, J. R. (2017). Bio-protocol 7(22): e2610.
DOI: 10.21769/BioProtoc.2610.
Visual demonstration of mounting gelatin embedded brain slices in a weigh boat filled with 0.12% w/v gelatin. In this example, only one of four vials was used for mapping antibody-mediated toxin deletions (each slice is 240 μm apart) and sequentially arranged from top left, moving along top to bottom, left to right.
Extracted from protocol
A Streamlined Method for the Preparation of Gelatin Embedded Brains and Simplified Organization of Sections for Serial Reconstructions
Liu, A. W., Aoki, S. and Wickens, J. R. (2017). Bio-protocol 7(22): e2610.
DOI: 10.21769/BioProtoc.2610.
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Keywords
Gelatin embedding, Serial reconstruction, Slide mounting, Lesion, Viral expression, Neuronal tracing
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