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Critical steps of the Fluorescent in situ hybridization (FISH) protocol.
DOI: 10.21769/BioProtoc.v110 Published: Nov 20, 2017 Views: 7263
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We show the critical steps for the FISH technique described in this protocol as follows: boil the slides with the antigen retrieval solution, place slides on ACD EZ-slide holder/rack into the HybEZTM Oven, create a barrier around tissue sections, add 60-100 μl per probe or amplification solution to each section, move the slide rack up and down, and acquire representative confocal images.
Extracted from protocol
Combination of Fluorescent in situ Hybridization (FISH) and Immunofluorescence Imaging for Detection of Cytokine Expression in Microglia/Macrophage Cells
Lanfranco, M., Loane, D. J., Mocchetti, I., Burns, M. P. and Villapol, S. (2017). Bio-protocol 7(22): e2608.
DOI: 10.21769/BioProtoc.2608.
We show the critical steps for the FISH technique described in this protocol as follows: boil the slides with the antigen retrieval solution, place slides on ACD EZ-slide holder/rack into the HybEZTM Oven, create a barrier around tissue sections, add 60-100 μl per probe or amplification solution to each section, move the slide rack up and down, and acquire representative confocal images.
Extracted from protocol
Combination of Fluorescent in situ Hybridization (FISH) and Immunofluorescence Imaging for Detection of Cytokine Expression in Microglia/Macrophage Cells
Lanfranco, M., Loane, D. J., Mocchetti, I., Burns, M. P. and Villapol, S. (2017). Bio-protocol 7(22): e2608.
DOI: 10.21769/BioProtoc.2608.
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Keywords
in situ hybridization, RNAscope, Traumatic brain injury (TBI), Macrophages, Immunofluorescence, Microglia cells, Neuroinflammation, Cytokines
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