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The injection procedure.
DOI: 10.21769/BioProtoc.v105 Published: Oct 5, 2017 Views: 7403
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The video shows (1) The localization of the needle and test release of injection mix, (2) Localization of the target animal and placing it into position for injection by rotating the gliding table, (3) Adjusting the needle position to target the gonad, (4) Switching to the 40x objective and centralize the position of the needle, (5) Focusing on the syncytium of the distal gonad, (6) Moving the animal into the needle and injecting the injection mix, as well as three more examples for the procedure.
Extracted from protocol
Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection
Rieckher, M. and Tavernarakis, N. (2017). Bio-protocol 7(19): e2565.
DOI: 10.21769/BioProtoc.2565.
The video shows (1) The localization of the needle and test release of injection mix, (2) Localization of the target animal and placing it into position for injection by rotating the gliding table, (3) Adjusting the needle position to target the gonad, (4) Switching to the 40x objective and centralize the position of the needle, (5) Focusing on the syncytium of the distal gonad, (6) Moving the animal into the needle and injecting the injection mix, as well as three more examples for the procedure.
Extracted from protocol
Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection
Rieckher, M. and Tavernarakis, N. (2017). Bio-protocol 7(19): e2565.
DOI: 10.21769/BioProtoc.2565.
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Keywords
Caenorhabditis elegans, Genetic transformation, Microinjection, Extrachromosomal arrays, Germline, GFP, Transgenic animals
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