Improve Research Reproducibility A Bio-protocol resource

Hi, May I know if this SUnSET assay works in HEK293T cells which is already transfected with plasmid for expression for 24h?

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Pooja Rk Oct 15, 2026
protocol Protocol: SUrface SEnsing of Translation (SUnSET), a Method Based on Western Blot Assessing Protein Synthesis Rates in vitro

 If yes will puromycin 5hr treatment after 24htransfection will be enough to study the effect of my protein driven translation inhibition?
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