step G4, do you centrifuge the THP-1 cells and resuspend them in fresh medium, or directly transfer the cell-containing medium to the 12-well plate?

protocol Protocol: Protocol for Generation of Single-Gene Knockout in Hard-to-Transfect THP1 Cell Lines Using CRISPR/Cas9

I am following your THP-1 lentiviral transduction protocol and would like clarification on step 4. When you say “collect cells and plate in a new 12-well plate,” do you mean centrifuging the suspension cells and resuspending them in fresh medium, or directly transferring the cell-contai Read more Read less more less

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