How to prevent tissue displacement during OCT embedding for cryosectioning 400 µm organotypic brain slices?

I am establishing an ex vivo demyelination model using brain slices from 6-8-week-old mice. The 400 µm vibratome slices are cultured for 10 days on membrane inserts (air-liquid interface) to assess myelin basic protein (MBP) expression in the corpus callosum under different dru Read more Read less