Improve Research Reproducibility A Bio-protocol resource

Which are the real times for each step?

AT
Anna Torrent May 21, 2026
protocol Protocol: Morphological Evaluation of Wound Healing Events in the Excisional Wound Healing Model in Rats
  1. Dehydration
  2. 90% ethanol: 30 s
  3. 100% ethanol I: 30 s
  4. 100% ethanol II: 30 s
  5. Clearing (Diaphanization)
  6. Xylol I: 30 s
  7. Xylol II: 30 s
  8. Wax infiltration (Inclusion in paraffin) in a regulated oven at 59 °C
  9. Paraffin I: 30 s Read more Read less more less
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