Considerations for improving the protocol

u1
user 1546637
5 days ago
protocol Protocol: Protocol for the Isolation and Analysis of Extracellular Vesicles From Peripheral Blood: Red Cell, Endothelial, and Platelet-Derived Extracellular Vesicles

The fluorescence minus one (FMO) control show notable staining for CD41, CD62-P, and CD62-E. This may pose a challenge when a specific extracellular vesicle (EV) subset is present at low abundance in the sample. Is it because of the no-wash protocol contributing to increased autofluores Read more Read less more less

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