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How do you differentiate nuclear vs ER presence of compounds?

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user 1483678 Nov 10, 2025
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Yuhang Wang Answered Nov 12, 2025

South China University of Technology

Use organelle-specific fluorescent markers (e.g., DAPI or H2B-GFP for nucleus, calnexin or ER-Tracker for ER) and colocalization imaging to compare signal overlap.

Confirm localization with confocal z-stacks or super-resolution microscopy, optionally supported by fractionation or immunostaining.

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