How should the ratio of the two plasmids be coordinated?

WC
Wang Cheng
Oct 9, 2025
protocol Protocol: Dual-Luciferase Transient Expression Assays of Arabidopsis Protoplasts

Thank you very much for sharing the details of your experiment. I have a question regarding the transfection step after protoplast isolation. In my current setup, I use a reporter plasmid where the promoter drives both LUC and REN, and an effector plas Read more Read less more less

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