Could you please advise on how to improve the penetration of Bodipy dye into brain slices

Hello Professor, I encountered a problem in the experiment involving co-staining of Bodipy and IBA1 and wanted to seek your guidance. I followed staining steps similar to yours, but the Bodipy dye penetrated the mouse brain slices only shallowly. As a result, when imaging with a fluores Read more Read less