How EV-depleted FBS can affected the trypsinization process?

protocol Protocol: Isolation of Extracellular Vesicles Derived from Mesenchymal Stromal Cells by Ultracentrifugation

Hi :) I will use EV-depeleted FBS in the step where I want to isolate EVs... the question is, how I do trypsinization process with trypsin and the inactivate trypsin without comprise my cells and using EV-depleted FBS...

Thank you so much.

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