what is the standard buffer for extraction protein from native page?

liu xiangyang
liu xiangyang
May 12, 2025
protocol Protocol: Purification of a Protein Exhibiting Isoleucine 2-epimerase Activity from Lactobacillus otakiensis JCM 15040
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Yuta Mutaguchi Author Answered May 21, 2025

Department of Biotechnology, Akita Prefectural University, Japan

Sorry for the late reply.

At that time, we used 50 mM sodium phosphate buffer (pH 7.2) containing 1 mM EDTA and 1 mM dithiothreitol as the standard buffer.

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