Should J-lat cells first be cell sorted for GFP- cells before treatment with compound and FACs?

Hi,

I'm currently a student who wants to use this protocol for my research to determine if my compounds reactivate J-Lat 10.6 cells out of latency. My question is whether we should first do a cell sorting by identifying all GFP-cells. And then used these GFP-cells, trea Read more Read less