Discrepancy in NaCl Concentration Between 2x and 1x HS Buffer in Protocol #3424

Hira Rafi
Hira Rafi
Apr 17, 2025
protocol Protocol: SarkoSpin: A Technique for Biochemical Isolation and Characterization of Pathological TDP-43 Aggregates

Hello,


In the 2x HS buffer described in this protocol, the NaCl concentration is listed as 300 mM, which makes sense. Howeve Read more Read less more less

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Florent Laferrière Author Answered Apr 18, 2025

Department of Quantitative Biomedicine, University of Zurich,

Thank you for your question,

Yes it's a typo, and the 1X concentration is indeed 150mM.

Good luck with your experiments.


Best,


Florent Laferriere

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