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I need to do calcein double-fluorescence labeling to determine...
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Gary ZT Lyu Sep 16, 2022
I need to do calcein double-fluorescence labeling to determine dynamic bone formation and flow cytometry to determine the number of immune cells in bone marrow. Can the bones used for calcein double fluorescent labeling and the bone marrow used to prepare single-cell suspensions come from Read more 
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Natalie Ann Sims Author Answered Sep 19, 2022
St. Vincent's Institute of Medical Research Melbourne
Hi Gary,
Calcein doesn't bind to cells, but chelates calcium, so should not cause a problem for FACS. We routinely supply calcein and collect bones for histology and carry out FACS analysis of marrow from the same animals.
Best of luck for your work.
Natalie
Calcein doesn't bind to cells, but chelates calcium, so should not cause a problem for FACS. We routinely supply calcein and collect bones for histology and carry out FACS analysis of marrow from the same animals.
Best of luck for your work.
Natalie
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