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Hello, in steps A-7, is it incubation at 100 °C, as his precondition...

罗旺 Sep 1, 2022

Protocol: Extraction and Quantification of Cyclic Di-GMP from Pseudomonas aeruginosa

Hello, in steps A-7, is it incubation at 100 °C, as his precondition is to wash with ice-cold pbs. If not, could you tell me what the temperature should be

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Karin Sauer Author Answered Sep 12, 2022

Biological Sciences Department, Binghamton University, State University of New York at Binghamton, USA

Both PBS and ethanol are kept on ice during the procedure. And yes, you will need to add ice-cold PBS to the pellet, resuspend, and then, transfer tube with ice cold solution to a heating block set to 100C.

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