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From literature, the NSCs are the compact cells (colonies).Are...
From literature, the NSCs are the compact cells (colonies).
Are the extensions from colonies (c, blue *) neurites? What are the single cells with these extensions (b, red *)?
Thank you for your time and guidance. It has helped tremendously.
Are the extensions from colonies (c, blue *) neurites? What are the single cells with these extensions (b, red *)?
Thank you for your time and guidance. It has helped tremendously.
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Ryutaro Kajihara Author Answered Aug 26, 2022
Department of Biomedical Laboratory Sciences, Faculty of Life Sciences Kumamoto University
Are the extensions from colonies (c, blue *) neurites?
> Yes, they look like neurites. If you want to differentiate the cells into neurons, you do not have to worry about these neurites and just proceed!
What are the single cells with these extensions (b, red *)?
> They look like flat non-neural cells. These cells do not have potency to differenciate.
If you observe a lot of these flat cells, you should "follow the following Steps C2a-C2g to purify the NSCs/NPCs from the non-neural cells" or "Just discard the cells and restart neural induction from scratch.
> Yes, they look like neurites. If you want to differentiate the cells into neurons, you do not have to worry about these neurites and just proceed!
What are the single cells with these extensions (b, red *)?
> They look like flat non-neural cells. These cells do not have potency to differenciate.
If you observe a lot of these flat cells, you should "follow the following Steps C2a-C2g to purify the NSCs/NPCs from the non-neural cells" or "Just discard the cells and restart neural induction from scratch.
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