How big are some of the largest complexes and could this method...

protocol Protocol: Von Willebrand Factor Multimer Analysis by Low Resolution SDS-Agarose Gel Electrophoresis
How big are some of the largest complexes and could this method be used to separate other large complexes?
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Peter L Turecek Author Answered Aug 25, 2022

R&D Global Medical Affairs, Baxalta Innovations GmbH, a Takeda company, Austria

Our agarose gels separate proteins and other macromolecules with molecular sizes up to 20 million Dalton.

Yes, the protocol cannot only be used for von Willebrand factor (VWF) for which we have optimized the conditions but also for other macromolecules. Recently our laboratory has investigated polymeric forms of recombinant human ADAMTS13 which is a glycosylated plasma protein that consists of a metalloproteinase domain, a disintegrin‐like domain, a thrombospondin type 1 (TSP1) repeat, a cysteine‐rich domain, a spacer domain, seven additional TSP1 repeats and two CUB domains, and it's complexes with recombinant VWF (https://pubmed.ncbi.nlm.nih.gov/31393047/). Although we haven't investigated it for all possible macromolecules, we need to assume that the protocol can be used for all molecules which migrate in an electric field in an agarose gel.
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