Hi,Why do we need to use glycine in step 14?

protocol Protocol: ImmunoFISH for Adherent Cultured Mammalian Cells
Hi,
Why do we need to use glycine in step 14?
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Francesca Rossiello Author Answered Aug 16, 2022

The FIRC Institute for Molecular Oncology, IFOM, Italy

Hi,

It's to block the fixation step, and it should also reduce the background fluorescence.

Best,

Francesca
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