I have 2 questions about this protocol. 1. Is pCPT-cAMP used...

Protocol: Glucose Production Assay in Primary Mouse Hepatocytes

I have 2 questions about this protocol.
1. Is pCPT-cAMP used for enhancing gluconeogenesis， and could glucagon be an alternative stimulator?
2. Why do we need to add insulin to the glucose production buffer since insulin would inhibit the process of gluconeogenesis?
Thank Read more Read less

Answer

Recommend

Follow

Share