Before you do any further experiments, make sure to cryo-freeze...

Hassan Hector Jul 7, 2022

Protocol: Generation of Stable Expression Mammalian Cell Lines Using Lentivirus

Before you do any further experiments, make sure to cryo-freeze about 1/4th portion of cells in 10% DMSO with DMEM in liquid nitrogen. Passage the rest of the cells for RNA/protein isolation, in order to validate for stable expression of protein of interest.
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