1) Excellent work. However, I am curious the yield (mg of protein)...

ARGHYA SETT
ARGHYA SETT
Jul 6, 2022
protocol Protocol: Heterologous Expression and High Degree Purification of the Restriction Endonuclease SauUSI
1) Excellent work. However, I am curious the yield (mg of protein) after size exclusion chromatography. If you have ever tried to scale up the process, what is the recovery of protein after affinity chromatography (His trap column).
2) Did you try with some other tagged protein (GST
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Vinayak Sadasivam Tumuluri Answered Jul 28, 2022

Brigham and Women's Hospital, Harvard Medical School

Thank you for your interest in our research. The yield for the protein after SEC is about 3 mg from a 4 litre culture. Previously, we tried expressing the protein with a cleavable CBP (intein) tag however, the expression (using small culture volumes) for this construct was very poor.
Best Regards,
Vinayak.
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