Dear author(s),You mentioned in the above protocol that after...
Dear author(s),
You mentioned in the above protocol that after you run the DNA sample on gel you would cut the correct band out then elute "DNA" with 0.3M NaCl. Would you be able to provide more details on how you elute DNA from gel using 0.3M NaCl?
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To elute the DNA efficiently, the gel must be crushed really small.
After cutting the DNA band from the gel, the gel was broken into small pieces. 0.3 M NaCl solution (more than 5 volumes of gel) was added to the tube. The DNA was eluted by shaking the tubes overnight.
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