Dear author(s),You mentioned in the above protocol that after...

Mahmoud Abdelatti
Mahmoud Abdelatti
Jul 5, 2022
protocol Protocol: Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies
Dear author(s),
You mentioned in the above protocol that after you run the DNA sample on gel you would cut the correct band out then elute "DNA" with 0.3M NaCl. Would you be able to provide more details on how you elute DNA from gel using 0.3M NaCl?
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Hiroshi Murakami Author Answered Jul 6, 2022

Nagoya University Aichi

To elute the DNA efficiently, the gel must be crushed really small.
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Hiroshi Murakami Author Answered Jul 6, 2022

Nagoya University Aichi

After cutting the DNA band from the gel, the gel was broken into small pieces. 0.3 M NaCl solution (more than 5 volumes of gel) was added to the tube. The DNA was eluted by shaking the tubes overnight.
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