Dear Dr. Gillotin, I tried this protocol for the separation...

RM
Rumpa Mahata
Jun 20, 2022
protocol Protocol: Isolation of Chromatin-bound Proteins from Subcellular Fractions for Biochemical Analysis
Dear Dr. Gillotin,
I tried this protocol for the separation of chromatin bound protein, but the isolation was not happened properly. Histone H3 was not detected in the chromatin part. Also there was tubulin contamination in the neuclear fraction. I have tried with both sonication bas
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