Dear Professor, I'd like to ask a little question about the dissolution of DPH in tetrahydrofuran. I bought the same tetrahydrofuran and DPH, but when adding tetrahydrofuran to DPH, I found that the two are not miscible and there is an obvious stratification phenomenon. The lower layer is Read moreRead less
Biochemistry and Molecular Biology, University Complutense , Spain, Spain,
Dear user. We have not seen any stratification phenomena in our preparations. In glasss tubes DPH crystals should dissolve easily in THF at a concentration of 1 mg/ml (4.3 mM). In any case, you can try to use more dilute (1-2 mM) stock solutions of DPH in THF. I hope you can solve this problem.