We are using the pBAC-s plasmid and not getting any colonies...

AS
AJ Singhal
Mar 18, 2022
protocol Protocol: Burkholderia glumae Competent Cells Preparation and Transformation
We are using the pBAC-s plasmid and not getting any colonies after transforming glumae. Any ideas?
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Hiromasa Saitoh Author Answered Mar 22, 2022

Tokyo University of Agriculture

We haven't used pBAC-s plasmid, but the transformation efficiency in Burkholderia glumae was varied depending on strains in our case. It might be worth trying to use several strains for transformation.
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