Dear authors, Have you tried other agars, like Bacto agar,...

protocol Protocol: Development and Standardization of Rapid and Efficient Seed Germination Protocol for Cannabis sativa
Dear authors, Have you tried other agars, like Bacto agar, in your MS solid medium? Or have to use Agar type E from Sigma? Thanks in advance.
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Aleksei Sorokin Author Answered Mar 4, 2022

Biological Sciences University of Lethbridge

Hi BODHISATTWA,

It is not necessary to use agar type E from Sigma, Bacteriological agar for example will work just fine for seed gemination purposes.
However, if you intend to use plant explants for plant cell culture/ tissue culture purposes, we recommend using type E agar for better results.

Best regards,
Aleksei Sorokin
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