Thanks for your detailed protocol. May I know how long the samples...

We haven't done long-term stability tests with the Stable Lyse/Stable Store buffers, but we have more experience with the first-generation Smart Tube buffer system (Proteomic Stabilizer, or PROT1). For PROT1, samples can be kept for a year or more at -80C with good results; but it's critical to avoid temperature fluctuations. Temporary transfer to -20C or frequent warming from freezer opening and closing have led to cell clumping. I don't know if this is equally true for Stable Lyse/Stable Store, but the latter definitely can be kept longer at room temperature prior to freezing (up to two weeks with minimal degradation).